Description
ROTIPHORESE® 10x TBE Buffer is a 10x concentrated buffer concentrate intended for electrophoresis workflows, especially horizontal agarose gel preparation and running conditions. Laboratories dilute the stock to working strength when casting agarose gels and filling electrophoresis tanks, where stable buffer composition is essential for reproducible separations. This ROTIPHORESE® preparation from manufacturer Carl ROTH fits molecular biology and analytical settings that routinely prepare agarose gels for horizontal electrophoresis. The one-litre unit quantity offers a practical stock volume for multiple gel batches and tank fills from a single bottle, reducing frequent reorder cycles for medium-throughput benches. Physical data provided for the concentrate include the stated density of 1,068 g/cm³ and boiling behaviour above 100 °C. Application focus covers electrophoresis, horizontal agarose electrophoresis, and preparation of agarose gels that depend on controlled ionic conditions from a reliable TBE concentrate.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Pack sizes: 1 l, 5 l, 2.5 l
- Density: 1,068 g/cm³
- Boiling point: >100 °C
- Storage temperature: +15 to +25 °C
- Product type: 10x TBE buffer concentrate
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
DangerHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTIPHORESE® 10x TBE Buffer, 10x conc. buffer concentrate, 1 l, glass
What is this article?
ROTIPHORESE® 10x TBE Buffer, 10x conc. buffer concentrate, 1 l, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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