Description
ROTIPHORESE® Sequencing gel is a ready-to-use buffer concentrate containing 50 % urea (w/v, 8,3 M) in 10× TBE buffer solution for denaturing polyacrylamide gel electrophoresis. With a density of 1,185 g/cm³ and a boiling point of 100 °C, it supports vertical electrophoresis workflows used in nucleic-acid sequencing and PAGE gel preparation. The concentrate is supplied in a 250 ml glass pack for convenient handling when casting sequencing gels. Part of the ROTIPHORESE® range from manufacturer Carl ROTH, it is intended for laboratories preparing denaturing PAGE gels where a urea–TBE matrix is required. Its ready-to-use format helps streamline gel-casting steps by delivering the urea and TBE components already combined in concentrated form. Suitable for molecular-biology and nucleic-acid analysis environments that rely on established sequencing-gel protocols, the product fits standard vertical electrophoresis (PAGE) preparation routines without additional formulation of the urea–buffer base.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Pack size: 250 ml glass
- Density: 1,185 g/cm³
- Boiling point: 100 °C
- Storage temperature: +4 to +15 °C
- Product type: Sequencing gel buffer concentrate
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
DangerHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTIPHORESE® Sequencing gel, buffer concentrate,, 250 ml, glass
What is this article?
ROTIPHORESE® Sequencing gel, buffer concentrate,, 250 ml, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
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The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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