Description
SDS-PAGE sample buffer concentrated (reducing) is a pharmacopoeia-quality laboratory reagent for preparing protein samples under reducing conditions for sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE). It is specified as Reag. Ph. Eur. in accordance with European Pharmacopoeia Chapter 4.1.1 under reagent number 1122100, and is grouped with reagents and volumetric standard solutions of pharmacopoeial quality. The concentrated reducing buffer is supplied in a 500 ml pack. A reducing SDS-PAGE sample buffer is combined with protein solutions so that disulfide bonds can be reduced and polypeptides coated with detergent before loading onto polyacrylamide gels; the concentrated format lets the laboratory set the working ratio. This reagent from manufacturer Carl ROTH belongs to the Ph. Eur. Reagents 4.1.1 assortment used in analytical and biochemical laboratories that require pharmacopoeia-referenced chemicals. The 500 ml unit, corresponding to a net weight of 0,700 kg, is suited to routine sample preparation for reducing SDS-PAGE.
Key features
- Pack size: 500 ml
- Density: 1,05 g/cm³
- Boiling point: 100 °C
- Quality: Reag. Ph. Eur.
- Ph. Eur. chapter: 4.1.1
- UNSPSC: 41116105
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
DangerHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about SDS-PAGE sample buffer concentrated (reducing)
What is this article?
SDS-PAGE sample buffer concentrated (reducing) is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
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The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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