Description
Trypsin is a crystalline proteolytic enzyme offering a minimum activity of 2500 USP-U/mg for reliable use in cell dissociation and related life-science workflows. Identified by CAS number 9002-07-7 and EG number 232-650-8, the preparation has an approximate molar mass of 24,000 g/mol. In cell biology laboratories, trypsin is routinely applied to release adherent cells from culture surfaces by cleaving peptide bonds in extracellular matrix and adhesion proteins, enabling gentle passaging of continuous cell lines and isolation of primary cells. Consistent specific activity supports reproducible detachment times and helps maintain cell viability when protocols are carefully controlled. This crystalline trypsin from manufacturer Carl ROTH fits standard tissue-culture and cell-harvest procedures in academic, biotech, and industrial settings. is practical for method optimisation, smaller culture volumes, and laboratories that prepare fresh working solutions as needed. Users typically dilute the enzyme in balanced salt solutions and terminate digestion with serum-containing medium or specific inhibitors according to their validated methods.
This article is part of Enzymes & substrates, in Biochemicals & reagents.
Key features
- Appearance: white to light brown hygroscopic powder
- Activity: ≥2400 USP-U/mg
- Loss on drying (60 °C): <5 %
- pH-value (1 %): 3.0-6.0
- CAS number: 9002-07-7
- Pack sizes: 100 mg, 1 g
- EG nr.: 232-650-8
- Molar mass: ~24,000 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
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Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about Trypsin, min. 2500 USP-U/mg, cryst., 100 mg, glass
What is this article?
Trypsin, min. 2500 USP-U/mg, cryst., 100 mg, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Enzymes & substrates, inside Biochemicals & reagents, in Microbiology lab supplies.
An enzyme is sold by activity, in units defined by a stated reaction, not only by milligrams. Source and specific activity are the specification. A substrate is the molecule that enzyme converts, often a chromogenic one so the reaction can be seen. Units from two suppliers are comparable only when the definition matches.
A biochemical is a molecule of life, sold as a reagent: an amino acid, a sugar, a nucleotide, a lipid, an enzyme, an antibiotic. The difference from a bulk chemical is that the use is a biological one, and the impurities that matter are the ones that change that use. An enzyme is specified by activity, not only by mass. An antibiotic is specified by potency. Papers are on PubMed. Structures are on PubChem. Strains and lines that consume these reagents are at ATCC.
Which category should I open next?
The category is Biochemicals & reagents. The sub-category is Enzymes & substrates. The catalogue is Microbiology lab supplies.
Shelves beside this one are Amino acids & peptides, Carbohydrates & sugars, Coenzymes, nucleotides & vitamins, Biochemicals (other), Antibiotics & antimycotics, Lipids, steroids & fatty acids and Detergents & surfactants.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.