Description
3,3',5,5'-Tetramethylbenzidine, dihydrochloride, min. 99 %, biochemistry, Molar mass (M) 313,27 g/mol, Storage temp. -20 °C, CAS No. 207738-08-7, EC No. 264-769-6, Empirical formula C16H20N2 · 2 HCl
Tetramethylbenzidine dihydrochloride is used as highly sensitive substrate for detection of horseradish peroxidase labelled probes, particularly in solution-based assays like ELISA techniques. It produces a soluble, blue-coloured end product, which can be read spectrophotometrically.
The dihydrochloride form is well water-soluble, making it a versatile tool in laborartory science.
Directions for use
Working solution: Immediately prior to use add 2 µl / 10 ml of 30 % hydrogen peroxide (Liem et al. (1979) Anal Biochem, 98:388-93).
Technical Information
| Enzyme | Horseradish peroxidase |
| Detection | Colour (blue) |
| Signal product | soluble (ELISA) |