Description
3,3',5,5'-Tetramethylbenzidine is an analytical-grade biochemical substrate supplied at min. 98 %, p.a. quality for laboratory use. It carries CAS number 54827-17-7, empirical formula C₁₆H₂₀N₂ and a molar mass of 240,35 g/mol, making it a well-defined reagent for chromogenic detection schemes in biochemistry and related life-science assays. Researchers and QC laboratories select high-purity tetramethylbenzidine when assay sensitivity and background control depend on a consistent p.a. grade material. The product is available from manufacturer Carl ROTH for biochemistry substrate applications where documented identity and minimum assay are required. Typical use includes enzymatic activity readouts, immunoassay signal generation and method development that relies on a classical TMB chromogen. Dissolve and handle under conditions appropriate to your validated procedure, protect solutions as required by your protocol, and refer to the safety data information for correct storage, PPE and disposal practices in the laboratory environment.
This article belongs on Protein biology (other), in Protein biology & Western blot.
Key features
- Appearance: beige cryst. powder
- Assay (GC): ≥99.0 %
- Sulphated ash: ≤0,2 %
- Loss on drying (3 h, 60 °C, vacuum): ≤1,0 %
- Melting range: 166-171 °C
- CAS number: 54827-17-7
- Pack sizes: 1 g, 5 g, 25 g
- EG nr.: 259-364-6
- Purity: min. 98 %, p.a.
- Empirical formula: C₁₆H₂₀N₂
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about 3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., 1 g, glass
What is this article?
3,3',5,5'-Tetramethylbenzidine, min. 98 %, p.a., 1 g, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein biology (other), inside Protein biology & Western blot, in Life Science & Microbiology.
This shelf is the protein-biology article that is not a membrane and not an extraction kit: stains for gels, buffers, small tools of a protein bench. The method, a gel stain or a quantitation assay, still chooses the bottle.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Protein biology (other). The catalogue is Life Science & Microbiology.
Shelves beside this one are Blotting membranes & papers and Protein extraction, purification & markers.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.